慢性阻塞性肺疾病患者外周血CD4^+CD25^+CD127^((Low/-))调节性T细胞的检测及临床意义

Significance of Detecting CD4^+CD25^+CD127^((Low/-)) T regulate cells in peripheral blood from Patients with Acute Chronic Obstructive Pulmonary Disease

目的:研究CD4+CD25+CD127(Low/-)调节性T细胞在慢性阻塞性肺疾病(COPD)急性发作期外周血中的比例改变及其临床意义。方法:以25例COPD急性发作期患者外周血为研究组,20名正常人外周血作为对照组,采用三色直接荧光素标记法和多参数流式细胞仪检测外周血CD4+CD25+CD127(Low/-)调节性T细胞的比例,同时检测外周血C-反应蛋白(CRP)、血沉(ESR)、免疫球蛋白(Ig)等水平。结果:COPD急性发作期患者外周血CD4+CD25+CD127(Low/-)调节性T细胞占外周血CD4+T淋巴细胞的比例明显低于健康对照组(P<0.01)。而COPD急性发作期患者外周血CRP、ESR、Ig等水平明显高于健康对照组(P<0.05)。COPD患者外周血调节T细胞下降与CRP和IgG升高成负相关。结论:COPD患者外周血CD4+CD25+CD127(Low/-)调节性T细胞在CD4+T淋巴细胞的比例明显减少,调节性T细胞等免疫调节因素可能在COPD的发病机制发挥重要作用。

Objective：To explore the role of CD4＋CD25＋CD127（Low/-） T regulate cells in peripheral blood in pathogenesis of Chronic Obstructive Pulmonary Disease（COPD）.Method：CD4＋CD25＋CD127（Low/-） T regulate cells of peripheral blood in 25 Patients with Acute Chronic Obstructive Pulmonary Disease and in 20 normal subjects was detected by fluorescein labeling method and flow cytometry,at the same time,the level of CRP,ESR and immunoglobulin in peripheral blood was detected.Results：The ratio of CD4＋CD25＋CD127（Low/-） T regulate cells and CD4＋ cells of peripheral blood in 25 Patients with Acute COPD was much lower than that of peripheral blood in normal subjects（P0.01）.And the level of CRP,ESR and immunoglobulin in peripheral blood in 25 Patients there was higher than that of peripheral blood in normal subjects（P0.05）.The percentage of CD4＋CD25＋CD127（Low/-） T regulate cells were negative correlated with CRP and IgG.Conclusion：CD4＋CD25＋CD127（Low/-） T regulate cells of peripheral blood in COPD Patients was diminished,T regulate cells possible play an important role in the pathogenesis of COPD.