三羟基异黄酮对肝纤维化大鼠血清和肝组织中TGF-β_1表达的影响

Effect of Genistein on TGF-β_1 in plasma and hepatic tissue of CCl_4-induced hepatic fibrosis rats

目的观察4,5,7-三羟基异黄酮(Genistein)对四氯化碳(CCl4)诱导的肝纤维化大鼠的防治作用及对转化生长因子β1(TGF-β1)表达的影响。方法采用CCl4造成大鼠肝纤维化模型,在实验的第10周末采血和取肝组织,检测血清中ALT、AST、TGF-β1和Ⅰ型胶原,HE染色法观察肝组织改变,免疫组织化学法检测肝组织TGF-β1表达。结果血清学检测显示Genistein能明显降低肝纤维化大鼠ALT、AST、TGF-β1和Ⅰ型胶原水平,病理组织学观察发现Genistein能明显改善肝纤维化大鼠肝组织结构和肝纤维化,免疫组织化学检测表明肝组织TGF-β1表达量与模型组比较显著降低。结论 Genistein对CCl4诱导的肝纤维化大鼠有较好的保护作用,对TGF-β1表达的影响可能是其作用机制之一。

Objective To investigate the antifibrotic effect of 4,5,7-Genistein on CCl4-induced liver fibrosis in rats and expression of TGF-β1. Methods A hepatic fibrosismodel was induced by CCl4.The levels of alanine transaminase （ALT）,aspartic transaminase （AST）,TGF-β1 and type-Ⅰcollagen in plasma were detected by chemical kit. The deposition of collagen was observed with HE staining. The expression of TGF-β1 in hepatic tissue was observed by immuno-histochemistry. Results The concentration of serum ALT,AST,TGF-β1 and type-Ⅰcollagen was significantly reduced in the middle and high dose treated groups compared with that of model group. A significant reduction of collagen deposition and rearrangement in Genistein -treated group was displayed in histopathological changes. The expression of TGF-β1 was considerably decreased in the treated animals. Conclusion Genistein has an obvious protective effect on model rats,and remarkably inhibits TGF-β1 expression in plasma and liver tissue which is thought to be one possible antifibrogenic mechanism.