基于微卫星位点的人工圈养豚鹿亲子关系鉴定

Paternity determination by seven microsatellite loci for the captive hog deer

豚鹿属我国国家I级重点保护动物,目前国内野生种群已经灭绝,人工圈养数量少,已极度濒危。成都动物园是国内最大的豚鹿饲养单位,对该园内豚鹿进行亲子鉴定及遗传谱系建立是我国豚鹿拯救工程中一重要环节。本文利用7个微卫星标记对成都动物园27只豚鹿个体进行了基因分型,在母本已知情况下成功鉴定了13对父子关系,其中排除法鉴定8对,似然法鉴定5对且置信度达95%。将亲子鉴定的结果辅以动物园豚鹿圈养的历史记录,我们构建了该园豚鹿的遗传谱系图。本文的研究成果将为后续人工繁殖中亲本雌雄配对的个体选择以及种群的遗传管理提供参考。

The hog deer （Axis porcinus） is a Class 1 National Key Protected Species in China; the species has been extirpated within China. China has a captive population of only about 30,of which the Chengdu Zoo has 22. It is critical for captive management within China to identify paternity relationships and establish the pedigree of the hog deer population in the Chengdu Zoo. We used 7 microsatellites to genotype 22 live and 5 dead hog deer individuals from the Chengdu Zoo; we identified potential 13 paternal relationships for animals in which maternity was already known. The exclusion method defined 8 of the 13 father-offspring pairs. The likelihood method determined the remaining 5 pairs with a confidence level of 95%. Combining the result of this paternity test with the breeding records of the Chengdu Zoo,we constructed the genetic pedigree for this hog deer population. Our result will serve as an important reference for the future artificial breeding strategy and management of the captive hog deer population in China.