呋塞米的电化学性质及伏安法测定

Electrochemistry property and the voltammetric determination of furosemide

目的:研究呋塞米的电化学性质,初步探讨呋塞米在玻碳电极上的反应机理,并用电化学方法对其进行检测。方法:在pH=4.0的醋酸-醋酸钠缓冲溶液中,用玻碳电极循环伏安法(CV)和差分脉冲伏安法(DPV)研究呋塞米的电化学性质,并用差分脉冲伏安法测定呋塞米的含量。结果:呋塞米的氧化峰电位为0.9V,氧化峰电流与呋塞米的浓度在6.0×10-7～3.2×10-4mol.L-1范围内呈良好的线性关系。该测定方法的检出限(S/N=3)为1.0×10-7mol.L-1。用标准加入法测定回收率范围在93.0%～98.4%,RSD为3.5%(n=4)。结论:呋塞米在0.9V处的氧化为1电子1质子参加的受扩散控制的不可逆过程。呋塞米的电化学测定方法能很好地用于实际样品的测定,而且结果与药典法一致。

Objective：To discuss the electrochemistry characteristic and the reaction mechanism on a glassy carbon electrode of furosemide. The concentration of furosemide was determined by the method of electrochemistry. Meth- ods ： In HAc - NaAc buffer solution （ pH = 4. 0 ）, the electrochemistry properties of furosemide were researched with a glassy carbon electrode by the cyclic voltammetry and the differential pulse voltammetry and the concentration of furosemide was detected by the differential pulse vohammetry. Results：The potential of furosemide oxidation peak was 0. 9 V and the peak currents were proportional to furosemide concentration in the range of 6.0 × 10 -7 - 3.2 ×10-4 mol · L-1 with the detection limit（S/N =3） of 1.0 × 10-7 mol · L-1. The range of recovery was 93.0% - 98.4% by the standard addition, and RSD was 3.5% （n = 4）. Conclusions：The anodic oxide of furosemide at 0. 9 V is irreversible diffusion - controlled and is characteristic of one electron and one proton. This method is applied in detection the content of furosemide sample and the results obtained by this method are in conformity with the results found by pharmacopoeia method.