摘要
本研究旨在提高基因转染的效率。试验使用聚乙烯亚胺(polyethylenimine,PEI)介导质粒pEGFP-N1转染NIH-3T3细胞,分为两组,分别进行一次转染和二次转染,于转染48h后在荧光倒置显微镜下观察。结果显示,部分细胞发出绿色荧光,二次转染组发绿色荧光的细胞明显多于一次转染组,且二次转染法可以明显提高基因的转染效率。
The study aimed at improving gene transfection efficiency. We used PEI-mediated plasmid pEGFP-N1 to transfect NIH-3T3 cells in this study. NIH-3T3 Cells were divided into two groups to conduct one and two transfection,and observed under fluorescent inverted microscope at 48 h after transfection. The results showed that some cells emited green fluorescence,and green fluorescent cells in two transfection group were more than one transfection group. Calculated transfection efficiency to showed that two transfection method could significantly improve gene transfection efficiency.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第5期82-84,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(30671509
30871791)
高等学校博士学科点专项科研基金资助项目(20061117004)
江苏省自然科学基金(BK2007077)
江苏省高校自然科学重大基础研究项目资助(08KJA230001)
关键词
基因
细胞
转染效率
gene
cell
transfection efficiency
