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藏猪肝X受体(LXRs)基因PCR-RFLP多态性分析 被引量:1

PCR-RFLP Polymorphisms of LXRs Genes in Tibet Pigs
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摘要 LXRα和LXRβ基因是猪肌肉发育和脂肪沉积的重要候选基因。本研究利用PCR-RFLP技术测定了112头藏猪的LXRα-BslI和LXRβ-AciI多态性。结果显示,藏猪群体中LXRα-BslI有CC、CG和GG3种基因型,基因型频率分别为0.4286、0.4643和0.1071;LXRβ-AciI有TT、TC和CC3种基因型,基因型频率分别为0.1964、0.5179和0.2857;与瘦肉型猪基因型分布差别较大。推测可能与藏猪肌肉和脂肪发育遗传特点有关。 The LXRα and LXRβ genes were main candidate genes for muscle growth and fat deposition. In the present study,the polymorphisms of LXRα-Bsl I and LXRβ-Aci I were measured with PCR-RFLP in 112 individuals of Tibet pig. The results showed that the LXRα-Bsl I in Tibet pig had 3 genotypes of CC,CG and GG,and frequents were 0.4286,0.4643 and 0.1071 respectively. The LXRβ-Aci I in Tibet pig had 3 genotypes of TT,TC and CC,and frequents were 0.1964,0.5179 and 0.2857 respectively. For the genotype distribution of the two genes Tibet pig was much different with lean-type pig breeds,which might be related to the special traits of muscle and fat developments in Tibet pig.
出处 《中国畜牧兽医》 CAS 北大核心 2010年第5期137-140,共4页 China Animal Husbandry & Veterinary Medicine
基金 国家科技支撑项目(2008BADB2B01) 转基因生物新品种培育科技重大专项(2009ZX08009-157B)
关键词 藏猪 LXRα基因 LXRβ基因 PCR-RFLP 多态性 Tibet pig LXRα gene LXRβ gene PCR-RFLP polymorphism
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