五味子醇提取物保肝作用成分分析

Analysis of Components of Alcohol Extracts of Shizandra against Hepatic Cell Injury

目的探讨五味子醇提物产生保肝作用的活性成分。方法建立原代肝细胞损伤模型,采用血清药效学实验证实五味子药效作用,高效液相色谱法及光谱分析含药血清中五味子化学成分,同时进行2种对照品的细胞药效学实验。结果模型组培养上清ALT,AST,MAO活性分别升高至(987.9±371.8)、(1695.4±600.5)、(46.7±6.3)nkat/L,MDA含量升高至(3.6±1.2)μmol/L,与正常组比较有显著性差异;五味子含药血清能有效地拮抗D-氨基半乳糖对肝细胞损伤,细胞上清液中ALT,AST,MAO,活性分别降低至(410.3±213.4)、(880.3±105.4)、(20.5±5.5)nkat/L、MDA含量降至(1.4±1.0)μmol/L,显著低于模型组;两种对照品能明显降低肝细胞损伤时所致升高的酶谱活性水平,对损伤的肝细胞有显著的修复和保护作用。结论醇甲、乙素是五味子阻滞肝细胞损伤的主要有效活性成分。

Objective To investigate the active components with pharmacodynamic effects in shizandra.Methods Liver cells were separated and the model of injured liver cells was established.The pharmacodynamic role of shizandra was demonstrated by pharmacodynamic test.The chemical components of shizandra were analyzed by high performance liquid chromatography chromatograms,and the pharmacodynamic effects of two control substances were observed by the test of cell pharmacodynamics.Results In the D-GlaN group active enzyme of ALT,AST,MAO and content of MDA rose respectively to（987.9 ±371.8）,（1695.4 ±600.5）,（46.7 ±6.3）nkat/L,（3.6 ±1.2）μmol/L,more remarkable the than in normal group.The drugserum in shizandra antagonized the injured liver cell induced by D-GlaN and the enzyme activity of ALT,AST,MAO and MDA content decreased respectively to（410.3±213.4）,（880.3 ±105.4）,（20.5 ±5.5）nkat/L and（1.4 ±1.0） μmol/L,notably lower than those in the model group.Two control substances markedly decreased the level of high enzyme activity that was induced by D-GlaN and there was obvious restoration and protection on the injured liver cells induced by D-GlaN.Conclusion Schizandrol A,Schisandrin B,are effective components of shizandra for retarding the injury of liver cells.