绿原酸对抗乙肝病毒-HBsAg和HBeAg的抑制作用

Inhibition of Chlorogenic Acid on HBsAg and HBeAg Secretion in Cultured Cell Line 2.2.15

目的观察绿原酸对2.2.15细胞分泌HBsAg、HBeAg的抑制作用。方法以2.2.15细胞为研究对象,通过细胞毒性实验确定绿原酸对细胞的最大无毒浓度,在最大无毒浓度以下将药物用培养液对倍稀释成250、125、62.5μg/ml,加入2.2.15细胞进行培养,每4d换同浓度药物培养液,分别收集第4、8天的培养液,酶联免疫法测定HBsAg、HBeAg含量。结果绿原酸对2.2.15细胞最大无毒浓度为250μg/ml。在最大无毒浓度(250μg/ml)时,两批试验对HBsAg、HBeAg均具有明显的抑制作用。第4、8天时对HBeAg的平均抑制率分别为89.45%、82.00%,对HBsAg的抑制率分别为86.54%、88.00%。结论绿原酸对2.2.15细胞分泌的HBsAg、HBeAg有显著的抑制作用。

Objective To observe the inhibition of chlorogenic acid（CA） on HBsAg,HBeAg in cultured cell line 2.2.15.Methods 2.2.15 cells were studied in vitro,The maximum non-toxic concentration was identified by cell toxicity experiments on CA.Below the maximum non-toxic concentration the drug was diluted into 250 μg/ml,125 μg/ml and 62.5 μg/ml used medium before 2.2.15 cells were treated.The medium was replaced by one of the same concentration every four days.The medium was collected at D4 and D8 and the content of HBsAg and HBeAg by ELISA was determined.Results The maximum non-toxic concentration of CA on 2.2.15 cells was 250 μg/ml and at this concentration the HBsAg,HBeAg were obviously inhibited.The inhibition ratio of HBeAg was 89.45% and 82.00% respectively at D4 and D8 and that of HBsAg was 86.54% and 88.00%,respectively.Conclusion CA could significantly inhibit the secretion of HbsAg and HBeAg on cultured cell line 2.2.15.