高效液相色谱法测定复方苦芩软膏中苦参碱的含量

Content Determination of Matrine in Compound Matrine and Baicalin Ointment by high performance liquid chromatography

目的建立复方苦芩软膏中苦参碱含量的测定方法。方法采用高效液相色谱法,色谱柱：SHISEIDO Cap-cell Pak-C18（4.6 mm×250 mm,5μm）;流动相：乙腈-0.1%磷酸溶液（5∶95）;流速：0.8 ml/min;检测波长：202 nm;柱温：40℃。结果苦参碱含量在0.499~2.994 mg（r=0.9999）范围内线性关系良好,平均回收率为101.47%,RSD为1.69%（n=6）。结论该方法操作简便、准确可靠,专属性、重复性好,可用于复方苦芩软膏的质量控制。

Objective To establish a method for determining matrine in Compound Matrine and Baicalin Ointment.Methods The highly effective liquid phase chromatography was used for the content of matrine.The chromatographic column was SHISEIDO Capcell Pak-C18（4.6mm×250mm,5μm）;the mobile phase was acetonitrile-0.1%phosphoric acid（5∶95）;the flow rate was 0.8 ml/min;the detective wavelength was 202 nm,and the column temperature was 40.0℃.Results The linear range of baicalin was 0.499～2.994 mg（r=0.9999）.The average recovery was 101.47% with a RSD of 1.69%（n=6）.Conclusion The method is simple,accurate and can be used for quality control of Compound Matrine and Baicalin Ointment.