利用稻谷壳水解液培养单细胞蛋白

Studies on the Technology of culture of Single Cell Pratein from the Hydrolysate of Rice Hull

以树状假丝酵母１．２５７为菌种，稻谷壳酸水解液为碳源，氨水中和水解液产物为氮源，在摇瓶条件下和１０Ｌ气升式发酵罐上进行发酵工艺条件的研究。结果表明，该菌种在初糖质量浓度２．５ｇ／ｄＬ，碳氮比３∶１，ｐＨ４．５～５．０的培养基中培养４８ｈ可获得较高的菌体蛋白量，在１０Ｌ发酵罐中试验，菌体干重为１２．６ｍｇ／ｍＬ，蛋白质含量为６２．８％，总糖利用率为８５．７％。

In this paper,the technology conditions of single cell protein culture was investigated under the shaking flask and in ten liter air lift fermentor with the Candida arborea in the culture medium containing rice hull hydrolysate as carbon source and product of hydrolysata with NH 3H 2O neutralization as nictrogeu source.The results showed that the more cell protein was obtained for culture 48 hours in culture medium in which initial content of sugar was 2.5% and ratio of carbon to nitrogen source was 3∶1 and pH was 4.5～5.0. The results of test in ten liter fermentor were 12.6 mg/ml of drycell and 62.8% of dry substrate protein content and 85.7% of sugar utilization.