丹参脂溶性成分的地域分布特点分析

Analysis on geographical distribution of liposoluble constituents in Salvia miltiorrhiza

目的分析全国不同产地丹参中脂溶性成分量的变化情况及其地域分布特点。方法采用HPLC法测定不同产地丹参脂溶性成分的量,运用SAS统计软件分析丹参脂溶性成分相关性及其地域分布特点。结果回归分析表明,丹参药材中丹参酮ⅡA量与二氢丹参酮Ⅰ量呈负相关,与其他两种呈正相关。聚类分析表明,3个野生药材与山东和河南大部分产地、四川中江集凤镇栽培药材脂溶性成分较高聚为一类。建议丹参药材中二氢丹参酮Ⅰ≥0.02%,丹参酮Ⅰ≥0.03%,隐丹参酮≥0.02%,丹参酮ⅡA≥0.2%。结论丹参的脂溶性成分呈现地域性差异,传统道地产区的量较高。丹参药材中二氢丹参酮Ⅰ≥0.045%,丹参酮Ⅰ≥0.12%,隐丹参酮≥0.10%,丹参酮ⅡA≥0.35%,才能符合历史上药用的要求。

Objective To analyze the content change of liposoluble constituents and their geographical distribution in Salvia miltiorrhiza from different habitats around ChinaMethods Determining the content of liposoluble constituents in S miltiorrhiza from different habitats by HPLC and using SAS to analyze correlation between liposoluble constituents in S miltiorrhiza and their geographical distributionResults Regression analysis showed that content of tanshinone ⅡA and dihydro tanshinoneⅠ presented negative correlation and positive correlation with the other two Cluster analysis showed that three wild medicinal herbs and the cultivated species in most habitats of Shandong and Henan Provinces and Zhongjiang in Sichuan Province are one kind Their contents of liposoluble constituents are higher, suggesting that dihydrotanshinone Ⅰ should be ≥ 0.02%, tanshinone Ⅰ ≥ 0.03%, cryptotanshinone ≥ 0.02%, and tanshinone ⅡA ≥0.02% Conclusion There are differences in liposoluble constituents in S miltiorrhiza between different habitats The contents are espesially higher in traditional and original location DihydrotanshinoneⅠ should be ≥ 0.045%, tanshinone Ⅰ ≥ 0.12%, cryptotanshinone ≥0.10%, and tanshinone ⅡA≥ 0.35%, which is consistent with the historical medical requirements.