HSP70基因多态性对煤工尘肺遗传易感性的影响

Effect of HSP70 gene polymorphism on genetic susceptibility of pneumoconiosis of coal worker

目的探讨职业人群中HSP70基因对煤工尘肺遗传易感性的影响。方法随机选取河南省某煤矿的工人200例,其中100例胸片结果判定为0+的为病例组,100例胸片结果判定为正常的为对照组。采用PCR-RFLP方法检测两组观察对象中HSP70的基因型分布情况。结果病例组HSP70-1b2/b2基因型的频率明显高于对照组(χ2=8.26,P<0.05),b2/b2基因型个体发生煤工尘肺的风险是b1/b1基因型个体的2.36倍(OR=2.36,95%CI=1.61～3.30);而HSP70-2和HSP70-hom的多态性在两组间差异无显著性。结论HSP70-1b2/b2基因型可能是煤工尘肺的一个易感基因型,存在该基因型的机体可能更容易罹患煤工尘肺。

Objective To explore the effect of heat shock protein 70（HSP70） gene polymorphism on genetic susceptibility of pneumoconiosis of coal worker（CWP）.Methods 100 workers whose sternites were assessed 0 ＋ were chosen as the case group and 100 workers as the control.HSP70 genotypes were analyzed by the polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique.Results There were significantly differences in HSP70-1 b2 /b2 genotype（χ2 = 8.26,P 0.05） between the case group and the control,the risks of CWP in the individuals with b2 /b2 genotype were 2.36 times than those with b1 /b1 genotype.The polymorphism of HSP70-2 and HSP70-hom had no statistics significance in the difference of the two groups.Conclusion The results suggested that the individual with HSP70-1 b2 /b2 genotype may be a predisposing genotype to CWP and the individuals with the genotype could have weaker adaptability than the others.