高效液相色谱法测定穿龙骨刺片中淫羊藿苷的含量

Determination of Icariin in Chuanlong Guci Pian by HPLC

目的采用高效液相色谱法测定穿龙骨刺片中淫羊藿苷的含量。方法采用外标一点法,Diamonsil ODS1 C18色谱柱,乙腈-水(27∶73)为流动相,流速为1.0mL/min,检测波长270nm。结果淫羊藿苷在0.125～1.00μg范围内呈良好线性,回归方程为Y=81601X+10356,r2=0.9997,平均加样回收率为99.12%,RSD=0.65%(n=6)。结论本方法简便、准确,专属性强,测定结果重复性好,为穿龙骨刺片中淫羊藿苷的定量分析提供了科学有效的方法。

Objective To establish the method for detemining the content of Icariin in Chuanlong Guci Pian by HPLC. Methods Diamonsil ODS1 C18 Column was used with acetonitrile-water （27∶73） as the mobile phase, detection wavelength as 270 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.125～1.00 μg for Icariin and linear equation was Y=81 601X＋10 356, r^ 2=0.999 7. The average recovery was 99.12% and RSD was 0.65% （n=6）. Conclusion This method was simple, accurate and proper, and the reduplication of the result was good, which provides scientific quantitative analysis of Icariin in Chuanlong Guci Pian.