摘要
目的建立磨盘草不同部位中鞣质含量测定方法。方法以没食子酸为对照品,采用磷钼钨酸-干酪素比色法测定鞣质含量。结果没食子酸浓度在2~10mg/L范围内与吸光度呈良好线性关系,平均加样回收率为99.63%,RSD=1.08%,磨盘草叶、茎、根中鞣质含量分别为0.34%、0.16%、0.27%。结论本方法灵敏,准确性强,精密度高,重复性好,适用于磨盘草中鞣质含量的测定。
Objective To establish the analytical methods for determining the contents of tannins in different vegetative organs of Abutilon indicum (L.) Sweet. Methods The content of tannins, expressed as gallic acid equivalents, was measured by using the phospho molybdenum tungstic acid-casein colorimetry. Results The good linearity between concentrations and absorbances of gallic acid was found in the range of 2~10 mg/L, the average recovery was 99.63% and RSD was 1.08%, the contents of tannins in leaves, stems and roots of Abutilon indicum (L.) Sweet were 0.34%, 0.16% and 0.27% respectively. Conclusion The proposed method is sensitive, precise, accurate and reproducible, and it is suitable for the determination of tannins in Abutilon indicum (L.) Sweet.
出处
《中国中医药信息杂志》
CAS
CSCD
2010年第3期53-54,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
海南省自然科学基金(808170)
关键词
磨盘草
鞣质
含量测定
Abutilon indicum (L.) Sweet
tannins
content determination