摘要
以eGFP为报告基因,生长激素释放激素(GHRH)为目的基因,利用精子介导的基因转移方法,将构建慢病毒载体质粒LV-EGFP-GHRH直接和猪精子孵育,再通过体外人工受精建立GHRH转基因猪模型。经PCR检测,52头后代中发现7头阳性个体,转基因效率为7.42%。本研究探索了应用精子介导的转基因方法将GHRH转移到动物体内,为猪的转基因育种提供了新的依据。
The transgenic pigs carrying GHRH gene were produced using the sperm-mediated gene transfer method.Lentiviral vector LV-EGFP-GHRH of GHRH as aim gene and EGFP as report gene was co-cultured with the sperm cells,and then fertilization in vitro was performed.Seven out of fifty-two offspring were found to be transgenic by PCR characterization carrying GHRH gene and the transgenic ratio was 7.42%.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2010年第5期693-697,共5页
Chinese Journal of Veterinary Science
基金
新品种培育重大专项基金资助项目(2008zx08006-004)
广东省科技厅农业攻关基金资助项目(2008B020100005)
关键词
猪
转基因
精子介导
GHRH
pig
transgenic
sperm-mediated gene transfer（SMGT）
GHRH
