宫颈癌cyclin G1表达与HPV分子亚型感染的研究

Investigation on expression of cyclin G1 and infection of HPV subtypes in cervical carcinoma

目的:探讨宫颈上皮内瘤变(CIN)和宫颈癌中细胞周期蛋白G1(cyclin G1)的表达与人乳头状瘤病毒(HPV)分子亚型感染的关系。方法:收集正常对照组(正常宫颈组织)20例、CIN组44例和宫颈癌组28例,用免疫组化二步法检测活检或术后石蜡包埋标本中cyclin G1表达水平,用导流杂交基因芯片技术检测术前宫颈脱落细胞HPV分子亚型。结果:在正常对照组、CIN组和宫颈癌组中,cyclin G1阳性表达率呈上升趋势,各组间两两比较,差异有统计学意义(P<0.05);HPV、高危型HPV、HPV16感染率也均呈上升趋势,各组间两两比较,差异有统计学意义(P<0.05);在宫颈癌组中,cyclin G1的表达率与高危型HPV感染率(r=0.382,P=0.045)、HPV16感染率(r=0.519,P=0.005)均呈显著正相关。结论:cy-clin G1在宫颈癌中的高表达,可能与宫颈癌的发生有关;宫颈癌中cyclin G1与高危型HPV呈显著正相关,可能通过协同作用导致宫颈癌的发生。

Objective： To explore the relationship between expression of cyclin G1 in cervical intraepithelial neoplasia （CIN） and cervical carcinoma and infection of human papillomavirus （HPV） subtypes. Methods： The expression levels of cyelin G1 in biopsy speci- mens or paraffin - embedded specimens after operation from 20 cases of normal cervical tissues （ control group） , 44 cases of CIN （ CIN group） and 28 cases of cervical carcinoma （cervical carcinoma group） were detected by immunohistochemical method, the HPV subtypes of cervical cast - off cells before operation were detected by flow - through hybridization and gene chip technique. Results： The positive rate of cyclin G1 expression showed a increasing trend in control group, CIN group and cervical carcinoma group, there was significant difference in multiple comparison （P 〈 0.05） ; the infection rates of HPV, high risk HPV and HPV16 increased, there was significant difference in multiple comparison （ P 〈 0.05 ） ; in cervical carcinoma group, there was a positive correlation between expression rate of cyclin G1 and infection rates of high risk HPV （y =0. 382, P =0. 045） and HPV16 （γ =0. 519, P =0. 005） . Conclusion： The high expression of cyclin G1 in cervical carcinoma may be related to oneogenesis of cervical carcinoma; there is a positive correlation between cyclin G1 in cervical carcino- ma and high risk HPV, and their synergy effect may induce the oncogenesis of cervical carcinoma.