cDNA基因芯片技术检测人卵巢癌组织表达差异基因

Detection of differently expressed genes in human ovarian carcinoma by cDNA gene chip technology

目的:探讨人卵巢癌组织表达差异基因,揭示卵巢癌发生、发展的分子机制。方法:2例人卵巢癌组织标本及2例正常人卵巢组织,取自第四军医大学西京医院妇产科手术切除标本。其中2例人卵巢癌组织标本经病理确诊低分化浆液性乳头状囊腺癌,临床诊断为卵巢癌Ⅲ期,临床诊断根据FIGO标准,选用2例正常卵巢组织为对照。提取卵巢组织中mRNA,提取并纯化卵巢癌组织中总RNA,应用cDNA基因芯片技术对共计588个基因进行表达差异基因检测。结果:cDNA基因芯片技术对卵巢癌组织和正常卵巢组织中588个基因同时进行比较,两组中共筛选出表达差异基因44条:其中11条表达上调基因(包括癌基因c-erbB2,neu,c-fos,c-mycproto-oncogenes,HER2 receptor等)、33条表达下调基因(包括RAR,MMP18,MMP19,p21,DNA-PK等)。结论:揭示人卵巢癌组织中表达差异基因,为早期诊断卵巢癌及判断预后提供理想标志物。

Objective： To explore the differently expressed genes, reveal the molecular mechanism of occurrence and development of ovarian carcinoma. Methods ： Two specimens of human ovarian carcinoma tissues and two specimens of normal ovarian tissues were select- ed from Department of Gynecology and Obstetrics. The two specimens of human ovarian carcinoma tissues were diagnosed as poorly differenti- ated serous cystadenocarcinoma by pathological examination and ovarian carcinoma of stage Ⅲ by clinical diagnosis according to International Federation of Gynecology and Obstetrics （FIGO） , the two specimens of normal ovarian tissues were selected as control group, mRNA in ovar- ian tissues was extracted, total RNA in ovarian carcinoma was extracted and purified. 588 genes were detected by cDNA gene chip technology for differently expressed genes. Results： The 588 genes in ovarian carcinoma tissues and normal ovarian tissues were compared by cDNA gene chip technology, 44 differently expressed genes were screened out in the two groups ： including 11 up - regulation genes （ c - erbB2, neu, c - fos, c - myc proto - oncogenes, HER2 receptor） and 33 down - regulation genes （ RAR, MMP18, MMP19, p21, DNA - PK）. Con- clusion： The differently expressed genes in human ovarian carcinoma are ideal markers in early diagnosis and judging prognosis.