大杯香菇辐射选育新株系子实体蛋白质构成的多元回归与聚类分析

Regression and cluster analysis on fruit bodies protein compost of Lentinus giganteus radiation strains

以20个大杯香菇品种和辐射选育新株系为材料,分析了大杯香菇品种和辐射选育新株系蛋白质及其构成的17种氨基酸含量间的多元相关、回归和聚类关系,并采用生物统计方法分析品种和新株系的蛋白质及各种氨基酸间的关系。结果表明:大杯香菇辐105号新株系子实体中天门冬氨酸、苏氨酸、甘氨酸、丙氨酸、胱氨酸、缬氨酸、异亮氨酸、苯丙氨酸、蛋白质含量最高,与部分品种和新株系子实体间差异显著或极显著,且大多数氨基酸间及其与蛋白质含量之间存在显著或极显著正相关关系;各种氨基酸与蛋白质含量之间分别有5种呈一次曲线关系,10种呈二次曲线关系,2种呈三次曲线关系;采用系统聚类的方法可将蛋白质及17种氨基酸含量聚为4个大类,对每类中的典型性状如精氨酸、亮氨酸、胱氨酸和蛋氨酸含量进行优化,可有效提高大杯香菇品质育种中蛋白质构成的改良效率。

Using 20 varieties and radiated mutants of Lentinus giganteus as materials,the multiple correlation,regression relation and cluster analysis between protein content and 17 amino acids contents were conducted to provide scientific basis for breeding of L.giganteus.The results show that among the new radiated mutants of L.giganteus,Fu105 contains the highest amounts of Asp,Thr,Gly,Ala,Cys,Val,Ile,Phe and protein in its fruit body,which is significantly higher than some tested varieties or new radiated mutants.There exists a significant positive correlation between most amino acid content and protein content,of which 5 amino acids have a linear,10 have conic,and 2 have cubic relationship with protein.Trough systematic cluster analysis,the protein content and 17 amino acids are divided into 4 groups.The optimization of typical characters,such contents of Arg,Leu,Cys and Met,in each group could improve the efficiency of protein composition in L.giganteus breeding.