高浓度大豆生防芽孢杆菌粉剂喷雾干燥微胶囊化工艺研究

Technology for micro-encapsulation of high density antifungal entophytic bacteria AF67 by spray drying

确定了微胶囊材料麦芽糊精和阿拉伯胶与大豆生防芽孢杆菌AF67具有良好的生物相容性。应用正交试验确定了生防菌喷雾干燥过程中影响菌粉收率、存活率、含水量的主要因素,并建立多元指数模型。模型确定的最佳工艺条件为:进风温度170℃,进料速度10mL/min,芯材与填充剂配比为1:8,固型物含量25%,活菌数达2.1×1010cfu/g。经验证,该数学模型具有可靠性。

This paper identified malt dextrin and arabic gum which have a good biocompatibility with Antifungal Entopbytic Bacteria AF67. The effect of inlet air temperature ,liquid feed rate, ratio of core material and the filler and solid content on the yield, survival rates and moisture content of AF67 powder during the spray drying were studied by the orthogonal experiment. The optimum spray drying conditions were as below： the inlet temperature was 170℃; the liquid feed rate was 10 mL/min; the ratio of core material and the filler was 1：8; the solid content was 25%, with the survival of 2.1×10^10cfu/g. The results of the validation experiment showed that these mathematical models were reliable.