天津野生白刺再生体系的建立

Regeneration System of Tianjin Wide N. tangutorum

以天津野生白刺为材料,通过不同激素、不同外植体、不同含量的MS培养基和赤霉素的筛选试验建立了白刺再生体系。结果表明:以初春时节白刺幼嫩茎段为外植体建立无菌系,以破坏生长点的茎尖为外植体进行再生;最适芽增殖培养基为MS+BA 3.0 mg/L+NAA 0.4 mg/L,最适增粗培养基为1/2MS+BA 1.0 mg/L+0.2 mg/L IAA,最适芽再生培养基是MS+BA 3.0 mg/L+NAA 0.4 mg/L+GA 3.0 mg/L,再生率为94%。最适伸长培养基为MS+BA 1.0 mg/L+IAA0.3 mg/L,最适生根培养基为1/2MS+IBA 0.5 mg/L。

The wild N. tangutorum of Tianjin was selected as the material of this experiment. According to the selection experiments of different concentrations of hormone, different explants, contents of MS culture media and gibberellin, the regeneration system was built up. The results showed that the early spring tender stems were the best explants for the asepsis system, and the damaged caudex tip were used as the explant to regenerate. The best multiplication culture medium was MS＋BA 3. 0 mg/L＋NAA 0. 4 mg/L, the best culture medium for aggrandizement was 1/2MS＋BA 1.0 mg/L＋ 0. 2 mg/L IAA,the best culture medium for regeneration was MS＋BA 3. 0 mg/L＋NAA 0.4 mg/L＋GA 3.0 mg/L, the regeneration rate was 94%. The best culture medium for elongation was MS＋BA 1.0 mg/L＋IAA 0. 3 mg/L,and the best culture medium for rooting was 1/2MS＋IBA 0.5 mg/L.