普通狗牙根种子组培技术优化及植株再生

Optimization of Common Bermudagrass Tissue Culture and Plantlet Regeneration

以普通狗牙根的成熟种子作外植体,通过胚性愈伤组织的发生进行植株再生。结果表明:诱导狗牙根种子产生愈伤组织的最佳培养基为:MS+2,4-D 3 mg/L+壳聚糖4 g/L;暗培养优于光培养,最高出愈率为87.0%;所诱导愈伤组织在1/2原激素浓度下继代,以MS+6-BA0.1 mg/L为分化培养基,获得了再生植株;半透明粘稠状愈伤组织的分化率最高,为63.1%。

A tissue culture system for plant regeneration of common bermudagrass was developed. Using mature seeds as experimental material, explant callus was produced in the dark on MS medium containing 2,4-D 3 mg/L and ehitosan 4 g/L. The highest frequencies of inducement was 87.0%. Maintaining the callus in the former conditions and medium, with the 1/2 level of 2,4-D. Then moved the to MS medium to obtain regenerated plantlets, with 6-BA 0. 1 mg/L. The highest regeneration frequency was 63.1%, result in the ropy translucency callus.