大叶风兰组培快繁技术体系的研究

Study on the Tissue Culture and Rapid Propagation Technique System of Big Leave Neoinetia falcata

通过大叶风兰的花梗腋芽诱导出无菌植株,再利用无菌植株茎尖诱导形成原球茎,成功地诱导分化形成了再生植株,筛选出了花梗腋芽启动、原球茎诱导、增殖、生根以及过渡培养等培养基配方,形成一套完整的大叶风兰组培快繁技术体系。

The aseptic plants were induced from flower stems axillary buds. The protocorms were induced from the shoot tips. Regenerated plants were formed under induction and culture on differentiated medium successfully. The flower stems axillary bud deriving,the protocorms inducing,regeneration,rooting,transition medium were selected. It formed a complete set of tissue culture and rapid propagation technique system of big leave Neoinetia falcata.