黄淮地区大豆种质资源对疫霉根腐病的抗病性评价

Resistance Evaluation of Soybean Germplasm from Huanghuai Valley to Phytophthora Root Rot

【目的】建立一套大豆抗疫霉菌根腐病基因鉴别菌系,并用于分析大豆品种(系)是否含有抗病基因Rps1a、Rps1c和Rps1k。【方法】采用下胚轴接种法测定了125个大豆疫霉菌分离物的毒性组成,筛选了不同毒力公式的6个大豆疫霉菌并建立了该菌系,测定了黄淮地区55个主栽大豆品种(系)对该菌系的抗性并通过基因推导方法进行抗病基因分析。【结果】55个大豆品种(系)共产生18种反应型。抗病基因的推导结果表明,有2个品种可能含有Rps1c,没有鉴定到可能携带有Rps1a或Rps1k的大豆品种(系)。【结论】黄淮地区携带抗病基因Rps1a、Rps1c和Rps1k的大豆品种(系)并不多,且易感疫霉菌,因此需要及时进行抗性育种并积极推广。

【Objective】 The aim of the study was to establish a set of differential strains to identify soybean resistance gene to Phytophthora root rot and then to apply it for analysis of the resistance genes Rps1a,Rps1c and Rps1k that soybean cultivars or lines may carry.【Method】 Virulence formula of 125 P.sojae isolates were determined using the hypocotyls inoculation technique,the strains,which include 6 isolates with different virulence formulas,were applied to identify the resistance of 55 soybean cultivars （lines） and resistance gene,were analyzed using the gene postulating procedure.【Result】 Eighteen reaction types occurred in 55 cultivars or lines and results of gene postulation indicated that 2 cultivars （lines） probably carried the gene Rps1c and no cultivar may carry the gene Rps1a or Rps1k.【Conclusion】 A few of soybean cultivars （lines） from Huanghuai valley carry Rps genes Rps1a,Rps1c and Rps1k and tend to be infected by Phytophthora sojae,so resistant cultivars （lines） need to be bred and popularized actively.