白藜芦醇干预对慢性心房纤颤猪心房组织中去乙酰化酶1表达的影响

The effect of resveratrol intervention on the expression of SIRT1 in the atrial tissues in pigs with chronic atrial fibrillation induced by right atrial tachypacing

目的探讨白藜芦醇(resveratrol,RES)干预对快速右房起搏诱发的慢性心房纤颤(atrial fibrillation,AF)猪心房组织中去乙酰化酶(SIRT)1表达的影响。方法18头小家猪(雌雄不拘)随机分为3组(n=6):起搏组(ATP组)、假手术组(sham组)和白藜芦醇干预组(RES组)。采用Seldinger血管穿刺技术穿刺右或左侧颈内静脉送入双极电极至右心房并连接至实验用起搏器(AOO),快速起搏心房(500次/min)2周(假手术组猪不起搏)制备慢性AF实验模型。RES组猪于起搏前1周开始服用RES(2.5 mg.kg-1.d-1)。RT-PCR分析SIRT1mRNA表达情况,Western blot法检测SIRT1蛋白表达水平的变化。结果RES组SIRT1 mRNA和蛋白的表达较ATP组和sham组均明显增加(P<0.05),ATP组稍高于sham组但差异无统计学意义(P>0.05)。结论RES干预可以明显增强快速起搏右房诱发的慢性AF猪心房组织中SIRT1的表达。

Objective To investigate the effect of resveratrol intervention on the expression of SIRT1 in the atrial tissues in pigs with atrial fibrillation（AF） induced by right tachypacing.Methods 18 pigs of both genders were randomized into 3 groups： atrial tachypacing（ATP） group（n=6）,resveratrol-intervened（RES） group（n=6） and sham group（n=6）.A bipolar electrode was inserted through the left or right internal jugular vein into the right atrial（RA） appendage using Seldinger technique,and was connected to experimental pacemakers（AOO） in subcutaneous pockets in the neck.The pigs in ATP group and RES group were paced at 500 beats per minute for 2 weeks to establish AF models.Resveratrol（2.5 mg·kg-1·d-1） was given orally in 3 divided doses,7 days prior to ATP onset and continued till the morning of the final open-chest experiment.The expression of SIRT1 mRNA was detected by RT-RCR and the variations in the level of SIRT1 protein expression was determined by Western blot.Results The expression of SIRT1 mRNA and protein in the RES group was markedly higher than that in ATP group and the sham group（P0.05）;and the expression of SIRT1 mRNA and protein in the ATP group was slightly higher than that in the sham group,but the difference had no statistical significance（P0.05）.Conclusion Resveratrol intervention can significantly enhance the expression of SIRT1 in the atrial tissues in pigs with chronic AF induced by right rapid pacing.