面包酵母添加光合细菌和Vc后培养轮虫的效果

Study on Effect of Bokers Yeast on Rotifer Cultivation

采用种群累积培养法，在控温３２℃条件下进行实验，观察在面包酵母中加入光合细菌和维生素Ｃ培养萼花臂尾轮虫的效果。结果表明，光合细菌对该轮虫的种群增长有明显的促进作用。在酵母液中加入１％～１０％的光合细菌液（菌液的浓度为１０×１０８～１３×１０８ｃｅｌ／ｍｌ），轮虫种群密度可增长４～５倍。维生素Ｃ的添加量在０．０２ｍｇ／ｍｌ时，对轮虫的种群增长有促进作用；在０．１ｍｇ／ｍｌ时，其促长作用显著；在本实验设计范围内，当浓度等于或高于０．５ｍｇ／ｍｌ时，由于极大地降低了培养液的ｐＨ值，导致轮虫死亡。

Experiments on effect of bakers yeast added with Photosynthetic Bacteria ( PSB ) and vitamin C ( Vc ) to culture rotifer, Brachionus calyciflorus, were conducted by the method of population accumulation culture and at temperature of 32℃. The results showed that PSB obviously accelarated population growth of the rotifer. If 1～10 percent of PSB ( concentration of bacteria liquor is 10×10 8～13×10 8 cell/ml ) were added to bakers yeast, population density of the rotifer was increased by 400%～500% over the control group. When the additive dosage of vitamin C was 0.02 mg/ml, accelaration of rotifer population growth could be observed. When the dosage reached 0.1 mg/ml, significant accelaration was found, when the dosage reached or exceeded 0.5 mg/ml, it could leed to the death of rotifer because of extremely reduction of pH value.