摘要
目的探讨凝胶抗人球蛋白试验的特点及其在溶血性疾病实验诊断中的作用。方法检测溶血性疾病患者127例,用Coomb′s微柱凝胶试剂卡对患者血液标本进行直接抗人球蛋试验(GDAT),以试管法抗人球蛋白试验作对照。凝胶法阳性者再用单特异性抗体IgG和C3d凝胶试剂卡进一步分型。结果试管法直接抗人球蛋白试验阳性17例,凝胶法阳性22例,两种检测方法有显著性差异(P<0.05)。对22份凝胶直接抗人球蛋白试验阳性标本进一步进行IgG和C3d分型,其中IgG+C3d型10例(45.2%),IgG型10例(45.2%),C3d型2例(9.6%)。结论微柱凝胶直接抗人球蛋白试验操作简便、重复性好,不需洗涤红细胞,灵敏度优于试管法,在溶血性疾病的实验室诊断中有较好的诊断价值。
Objective To study the diagnostic value of the micro-column gel Coomb＇s test in hemolytic diseases. Methods micro-coluum gel and direct antiglobulin test （DAT） were used to study 127 hemolytic anemia cases, for the positive test cases, anti-IgG and-C3d were used to further test the antibody classes. Results 17 positive were identified by routine DAT typing in 127 hemolytic cases but 22 positive cases were identified by Microcolumn Gel, incuding 10 IgG positive cases, 2 C3d positive and 10 both IgG and C3d mixed postitive cases. There were statistically differences between the micro-gel and routine DAT in the diagnosis of the diseases. Conclusion Micro-column gel Coomb＇s test is a sensitive and applicative method for the diagnosis of hemolytic diseases.
出处
《广州医药》
2010年第3期63-64,共2页
Guangzhou Medical Journal
