大鼠正畸牙根吸收修复早期BMP-2与BSP表达的研究

Study of BMP-2 and BSP in early reparative process of orthodontical tooth resorption in rats

目的观察SD大鼠正畸牙根吸收早期修复过程中牙周组织骨形成蛋白-2(BMP-2)、骨涎蛋白(BSP)的表达。方法选取48只雄性SD大鼠,正畸加力14 d后拆除矫治器,在装置拆除后分别在0、1、3、5、7、10、14、28 d处死,HE染色观察牙周组织形态学变化,用免疫组织化学法检测BMP-2、BSP的表达。结果 BMP-2、BSP的表达在矫治器拆除后开始升高,BMP-2在第1天时达到最高,BSP在第3天时达到最高,然后逐渐下降,到第28天时降至对照组水平。结论 BMP-2、BSP的浓度在牙周组织中选择性地表达升高,反映了矫治器拆除后牙周组织的修复过程活跃。

Objective To investigate changes of BMP-2 and BSP during cementum repair following experimentally induced tooth movement,and to characterize the cells taking part in the regenerative process of SD rat.Methods After 14 d treatment,the appliance was removed,48 male SD rats were killed in groups on 0,1,3,5,7,10,14,28 d.Routine histological staining for viewing changes of periodontium histomorphology,immunohistochemical staining for bone morphogenetic protein 2（BMP-2） and bone sialoprotein（BSP）were conducted.Results Expression of BMP-2 and BSP were increased notably immediately after withdrawing the coils.BMP-2 peaked on 1 d,BSP peaked on 3 d,then decreased to the level in the control group on 28 d.Conclusion The concentration of BMP-2 and BSP is preferentially expressed strongly intensity,which shows that the repair process is active after withdrawing force.