茶多酚对ACC-M细胞Fas/FasL、PCNA表达的影响

Influence of tea polyphenols on expression of Fas/FasL and PCNA in ACC-M

目的体外观察不同浓度茶多酚对腺样囊性癌肺高转移细胞株(ACC-M)生长、Fas/FasL和增殖细胞核抗原(PC-NA)表达的影响。方法 (1)不同浓度茶多酚(0.05、0.1、0.15、0.2 g/L)组处理对数期生长ACC-M细胞;(2)用MTT法观察不同浓度茶多酚对ACC-M细胞生长的影响;(3)用免疫细胞化学法检测不同浓度茶多酚对ACC-M细胞中Fas/FasL和PCNA表达的影响。结果 (1)茶多酚对ACC-M细胞生长抑制作用明显(P<0.05);(2)细胞免疫组化结果显示:与未加茶多酚相比,加入茶多酚后,在ACC-M细胞中Fas蛋白表达增高(P<0.05)、FasL和PCNA蛋白表达降低(P<0.05),且随着浓度的增加更为明显。结论茶多酚可抑制ACC-M细胞生长,这种抑制作用可能与茶多酚促进ACC-M细胞中Fas表达和抑制FasL、PCNA表达有关。

Objective To observe the influence of tea polyphenols（TP） on the growth and to analyze the expression of Fas/FasL and proliferating cell nuclear antigen（PCNA） of adenoid cystic carcinoma cell clones highly metastatic to the lung（ACC-M） in vitro.Methods（1）ACC-M was cultured to exponential phase of growth with different concentration（0.05 g/L,0.1 g/L,0.15 g/L,0.2 g/L）of TP.（2）The influence of TP on the growth of ACC-M was measured by MTT.（3）The protein expression of Fas/FasL and PCNA was examined by immunohistochemical method and quantitatively analyze by immunohistochemical assay.Results（1）TP effectively inhibited the growth of ACC-M in a concentration-dependent manner（P0.05）.（2）The expression of Fas increased but FasL and PCNA declined after stimulated by TP by immunocytochemistry.It was more obvious with the increase of the concentration.Conclusion TP could up-regulate the expression of Fas and down-regulate the expression of FasL and PCNA,which maybe contribute to inhibit the growth of ACC-M in vitro.