摘要
建立一种测定乳制品中纳他霉素含量的高效液相色谱法。样品经甲醇萃取后进样,采用C18250mm×4.6mm色谱柱分离,流动相为甲醇:水=6∶4(体积比),紫外检测;A-R303nm,RSD为0.56%～0.82%(n=5),标准曲线R=0.9998,平均回收率分别为97.89%,最低检出限为0.03mg/kg。
Determination of the establishment of a dairy Natamycin content in the high-performance liquid chromatography. The sample was less like methanol extraction, using C18 250 mm×4.6 mm separation column, mobile phase was methanol： water=6∶4（volume ratio）,UV detector; A-R303nm, RSD 0.56 %-0. 82 %（n=5）, standard curve,R=0.999 8, average recoveries were 97.89 %, the minimum detection limit of 0.03 mg/kg.
出处
《食品研究与开发》
CAS
北大核心
2010年第4期111-112,125,共3页
Food Research and Development
关键词
高效液相色谱
纳他霉素
乳制品
high-performance liquid chromatography
Natamycin
dairy products
