环氧化聚苯乙烯的制备及其在果胶酶固定化中的应用

Studies on Preparation of Epoxy Polystyrene Sphere and Its Application of Immobilization of Pectinase

将环氧聚醚接枝于胺化的聚苯乙烯微球,制备环氧化聚苯乙烯微球,研究胺化聚苯乙烯微球的溶胀时间、反应体系、反应时间、温度等因素对微球环氧化的影响。结果表明,该反应的最佳条件:反应溶剂用二氧六环,反应温度80℃,反应时间25 h,溶胀时间4 h,胺基担载量2.1 mmol NH2/g时反应的环氧基固载量达到最大,最大环氧基担载量可达1.59 mmol/g。并用该环氧微球作为载体,对果胶酶进行固定化,研究微球吸附温度、吸附pH值、吸附时间对果胶酶固定化效果的影响,得到活力较高的固定化果胶酶,酶活力回收率约为56.4%,最适反应温度由游离酶的50℃上升至65℃,最适pH由游离酶的3.5升至4.5,Km值由游离酶的5.14 g/L降低为3.11 g/L。操作稳定性很强,连续使用10批次,酶活仍为77%。

In this paper,epoxy PS micro-sphere is synthesized by amino polystyrene sphere and polyethylene glycol bisepoxypropyl.The effect of the amino loading of the amino polystyrene micro-sphere,the dissolved time of micro-sphere,reaction solvents,time,temperature were studied and optimized the reaction.The results show that the best response conditions： dioxane liquid,80 ℃ for 25 hours,swelling for 4 h,the amino loading of the amino polystyrene micro-sphere is 2.1 mmol NH2/g.In this condition,the maximum amount of immobilized Epoxy group of the amino polystyrene micro-sphere is 1.59 mmol/g.And we immobilized pectinase with this production,the activity recovery rate of the immobilized pectinase is about 56.4 percent,In comparison with the free enzyme,the optimum temperature is shift from 50 ℃ to 65 ℃,optimum pH from 3.5 to 4.5,the Km value of the immobilized enzyme and free enzyme were 3.11 g/L and 5.14 g/L,respectively.The pectinase immobilized with this new carrier showed a good operational stability.The recovery activity of the immobilized pectinase was more than 77 % after repeated 10 times.