摘要
为了探明水稻对条纹叶枯病的抗性遗传机制,定位相关QTL,以感病保持系66B和抗病粳稻恢复系C161构建的DH群体为材料,利用人工接种技术,以各世代对条纹叶枯病的病情指数比率为表型值,对C161的抗病性进行遗传分析。结果表明:该群体对条纹叶枯病的抗性遗传由3对主基因加多基因控制,符合加性主基因+加性-显性多基因模型(G-2),利用Qgene软件采用复合区间作图法检测到3个与抗病相关的QTL,分别位于第1,9,11染色体上RM283-RM259、RM257-RM160和RM287-RM229标记区间内,LOD值分别为3.798,3.942和4.965;对表型变异的解释率分别为16.7%、17.2%和21.2%,分离分析的结果与QTL定位的结果相对一致,可相互印证。
In order to probe resistant mechanism and map QTLs for the resistance to rice stripe,using the DH population from a cross of 66B ×C161,the present study estimated the genetic model of rice stripe resistance using the artificial inoculation technique to induce the rice stripe,and phenotypic value scored based on disease index ratio to rice stripe of every generation.The results showed that rice stripe resistance was controlled by three major genes and polygene（G-2 model）.With composite interval mapping under Qgene,three QTLs associated with rice stripe resistance were identified,which located in RM283-RM259,RM257-RM160 and RM287-RM229 on chromosome 1,chromosome 9 and chromosome 11 respectively.The QTLs＇ LOD value were 3.798,3.942 and 4.965,which explained 16.7%,17.2% and 21.2% of the total phenotypic variation.The results from segregation analysis and QTL mapping appeared relatively consistent and could be verified each other.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2010年第1期3-7,共5页
Journal of Shenyang Agricultural University
基金
农业部农业科技跨越计划资助项目(2007GB00060)
辽宁省自然科学基金项目(20092207)
