卡托普利对压力负荷增加大鼠心肌α-MHC和β-MHC mRNA表达的影响

Effects of captopril on the expression of myocardial α-myosin heavy chain and β-myosin heavy chain mRNA in rat with pressure overload

目的观察卡托普利对压力负荷增加大鼠心肌α-MHC和β-MHC mRNA表达的影响,探讨卡托普利逆转心肌肥厚的作用机制。方法 36只雄性SD大鼠,随机分为假手术组、模型组、卡托普利组。采用RT-PCR的方法检测大鼠心肌组织中α-MHC、β-MHC基因表达。结果模型组大鼠左室心肌组织α-MHC mRNA表达较假手术组下降(P<0.05),卡托普利组左室心肌组织α-MHC mRNA表达较模型组显著升高(P<0.05)。模型组大鼠左室心肌组织β-MHC mRNA表达较假手术组升高(P<0.05),卡托普利组左室心肌组织β-MHC mRNA表达较模型组下降(P<0.05)。结论卡托普利增加α-MHC基因表达,降低β-MHC基因表达,可能与其逆转心肌肥厚作用相关。

Objective To observe the effects of captopril on the expression of myocardial α-myosin heavy chain （α-MHC） and β-myosin heavy chain （β-MHC） mRNA in rat with pressure overload,and to explore the mechanism of the regression of left ventricular hypertrophy. Methods Thirty-six SD male rats were randomized into sham operation group,model group,and captopril group.The model rats were established by pressure overload.RT-PCR was used to detect the expression of α-MHC and β-MHC in myocardial tissues. Results The α-MHC mRNA expression in left ventricular myocardial tissue was lower in model group than that in sham operation group （P〈0.05）.The α-MHC mRNA expression in captopril group was higher than that in model group （P〈0.05）. The β-MHC mRNA expression of left ventricular myocardial tissue was higher in model group than that in sham operation group （P〈0.05）. The β-MHC mRNA expression was lower in captopril group than that in model group （P〈0.05）. Conclusion Captopril could increase the expression of α-MHC and decrease the expression of β-MHC,which may be one of mechanisms for regression of myocardial hypertrophy.