小鼠血浆中三七皂苷C质量浓度的测定方法

Determination Method for Notoginsenoside C in Mouse Plasma

给出了血浆中三七皂苷C质量浓度的测定方法:采用甲醇提取法从血浆中提取三七皂苷C,以原人参二醇为内标物;采用RP-HPLC法测定质量浓度,用ODSC18色谱柱(4.6mm×250mm,5μm),以V(甲醇)∶V(水)=90∶10为流动相,流速1.0mL/min,检测波长203nm.结果表明:三七皂苷C和原人参二醇的保留时间分别为13.6min和23.6min,色谱峰之间达到基线分离,且不受空白血浆中其他成分的干扰;日内精密度RSD均小于4.38%,日间精密度RSD均小5.22%,方法回收率为95.2%～99.1%.

To establish a method to determine the concentration of notoginsenoside C in plasma,a variety of methods were examined in the experiment to try to detect the mass concerntration of notoginsenoside C in plasma,and finally the method of extraction with methanol with protopanaxadiol as the internal standard was confirmed the best. The RP-HPLC method was estabilished and the conditions were ODS C18 column （4.6 mm×250 mm,5 μm）,the mobile phase of methanol-water （V（methonol） ：V（water）=90 ：10）,flow rate of 1.0 mL/min,and detection wavelength 203 nm. The retention time of notoginsenoside C and that of protopanaxadiol were respectively 13.6 min and 23.6 min. The peaks of samples were seperated well from each other without being interrupted by other components peaks. RSD of daily precision was less than 4.38%,RSD of inter-day precision was 5.22%,and the method recovery was in a range from 95.2% to 99.1%.