云南会泽废弃铅锌矿区和非矿区中华山蓼根际真菌镉耐性研究

Cd Tolerance of Rhizosphere Fungi of Oxyria sinensis Hemsle in Abandoned Lead-zinc Mining and Non-mining Area in Huize,Yunnan,China

采用常规、含Cd2+和含Pb2+的马丁氏培养基,对云南省会泽县废弃铅锌矿区和非矿区中华山蓼(Oxyria sinensis Hemsle)根际真菌进行分离,将分离的菌株接种到含不同浓度(0、0.05、0.5和5mmol·L-1)Cd2+的马铃薯葡萄糖培养液中,研究和比较了废弃铅锌矿区和非矿区中华山蓼根际真菌的镉耐性。结果表明,Cd2+显著抑制铅锌矿区和非矿区中华山蓼根际真菌的生长;Cd2+对常规、含Cd2+和含Pb2+的马丁氏培养基分离的铅锌矿区中华山蓼根际真菌的EC50平均值和最大值均明显大于非矿区,表明铅锌矿区中华山蓼根际真菌对Cd2+的耐性强于非矿区,采用含Cd2+培养基,从铅锌矿区中华山蓼根际分离出的真菌Cd2+耐性最强。

Culturable rhizosphere fungi of Oxyria sinensis Hemsle in abandoned lead-zinc mining and non-mining area in Huize,Yunnan,China were isolated with Martin＇s medium which were normal,Cd2＋ and Pb2＋ contained.Cd2＋ tolerance of those isolated fungi were investigated with PDA fluid media containing 0,0.05,0.5 and 5 mmol·L-1 Cd2＋.The results showed that：the growth of rhizosphere funi of Oxyria sinensis Hemsle was significantly suppressed by Cd2＋.The mean and maximum EC50 value of Cd2＋ of rhizosphere fungi of Oxyria sinensis Hemsle in abandoned lead-zinc mining area isolated by normal,Cd2＋ and Pb2＋ containing media was obviously higher than that in non-mining area,which meant Cd tolerance of rhizosphere fungi of Oxyria sinensis Hemsle in abandoned lead-zinc mining area was more than that in non-mining area.Among these fungi isolated by these 3 media,rhizosphere fungi of Oxyria sinensis Hemsle in abandoned lead-zinc mining area isolated by Cd2＋ containing media were the most tolerant for Cd.