胶束电动毛细管色谱法测定酸枣仁皂苷A和B(英文)

Determination of Jujubosides A and B by Micellar Electrokinetic Capillary Chromatography

目的:建立测定酸枣仁皂苷A和B的新方法。方法:采用胶束电动毛细管色谱法,熔硅毛细管尺寸60cm×75μm(有效柱长50cm);缓冲液组成为20mmol·L-1硼酸盐、60mmol·L-1胆酸钠和20%甲醇,pH8.2;检测波长203nm;重力进样高度22cm,进样时间7s;分离电压15kV;柱温25°C。结果:酸枣仁皂苷A和B的线性动态范围分别为30-900mg·L-1和40-1250mg·L-1(r2为0.9979、0.9969);检出限为5.68和11.86mg·L-1;900和1250mg·L-1时的精密度为0.46%和0.58%;平均加标回收率为98.1%、98.4%。结论:本方法可满足酸枣仁中皂苷A和B的测定要求,可作为酸枣仁药材的质量控制方法。

AIM：To develop a new method for the determination of jujubosides A and B.METHODS：Micellar electrokinetic capillary chromatography was used in the assay.The dimension of uncoated fused-silica capillary was 75 μm i.d.,60 cm total length and 50 cm effective length.The running buffer contained 20 mmol·L^-1 borate,60 mmol·L^-1 sodium cholate and 20 % methanol,and its pH was adjusted to 8.2.The gravity injection mode was used with the injection time of 7 s and the height difference of 22 cm.The separation voltage was 15 kV;the detection wavelength was 203 nm;the capillary temperature was 25 °C.RESULTS：The linear dynamic range was 30-900 mg·L^-1 for jujuboside A（r^2 = 0.997 9） and 40-1 250 mg·L^-1 for jujuboside B（r2 = 0.996 9）.The limits of detection were 5.68 mg·L^-1 and 11.86 mg·L^-1 for jujubosides A and B,respectively.The precisions were 0.46% for 900 mg·L^-1 jujuboside A and 0.58% for 1 250 mg·L^-1 jujuboside B.The average recoveries were 98.1% and 98.4% for jujubosides A and B.CONCLUSION：The assay can meet the requirement of determining jujubosides A and B,and can be applied to control the quality of the raw material,Semen Ziziphi Spinosae.