地衣芽孢杆菌De株对黄鳍鲷生长及其养殖池塘主要环境因子的影响

Effects of Bacillus licheniformis strain De on growth of Sparus latus and main environmental factors in aquaculture pond

运用综合对比分析法,对黄鳍鲷(Sparu slatus)养殖中定期泼洒地衣芽孢杆菌(Bacillus licheniformis)De的应用效果进行了探讨。结果表明,施用地衣芽孢杆菌De可在一定程度上优化水体环境和养殖生产性能,使养殖黄鳍鲷的成活率、体长增长率和体质量增长率分别提高18.2%、21.0%和312%;显著降低水中氨氮(NH3-N)、亚硝酸盐(NO2-N)、活性磷酸盐(PO43-P)质量浓度及底泥中有机碳的质量分数(P<0.05),其中养殖前、后期的底泥有机碳质量分数分别较对照组降低49.77%和22.63%,NO2-N、PO34-P质量浓度则总体较对照组降低25.82%和41.00%,NH3-N质量浓度在养殖前、中和后期较对照组降低36.33%、18.10%和14.28%;水体中弧菌数量在养殖后期较对照组降低61.76%,底泥中的总异养细菌数量在养殖中、后期较对照组提高38.61%,整个养殖期间水体及底泥中的芽孢杆菌数量分别较对照组提高15.34%和26.37%。

The effects of Bacillus licheniformis strain De in Sparus latus culture were studied by using comprehensive analysis approach. The results showed that the water environment and aquaculture performance were optimized by B. licheniformis strain De. The survival rate, length and weight growth rate of S. latus increased 18.2%, 21.0% and 312% respectively; NH3-N, NO2- -N and PO43-- P concentration in water and organic carbon in sediment decreased significantly （ P 〈 0. 05 ）. Compared with the control group, the organic carbon content in sediment decreased 49.77% and 22. 63% at earlier and later stages; NO2^- -N and PO4^3- -P concentration decreased 25.82% and 41.00% ; NH3-N decreased 36. 33% , 18.10% and 14. 28% at earlier, middle and later stages respectively; Vibrio concentration decreased 61.76% at later stage; the quantity of heterotrophic bacteria in sediment increased 38. 61% at middle and later stages, while that of Bacillus in water and sediment increased 15.34% and 26. 37% throughout the culture period.