HPLC法测定仙乐雄胶囊中淫羊藿苷的含量

Determination of Lcariin in Xianlexiong Capsules by HPLC

目的:建立仙乐雄胶囊中淫羊藿苷含量的测定方法。方法:采用HPLC法,ODSC18(4.6mm×250mm,5μm),以乙腈-水(30∶70)为流动相,流速1.0ml/min,检测波长270nm。结果:淫羊藿苷在(5.35-85.60)μg.ml-1的进样浓度范围内与其峰面积呈良好的线性关系(r=0.9998),平均回收率为98.06%(RSD=0.44%,n=6)。结论:本方法操作简便、灵敏、准确,可用于仙乐雄胶囊的质量控制。

AIM：To establish the content determination method of lcariin in Xianlexiong Capsules by HPLC. METHODS：HPLC analysis was carried out at the following conditions：Cromasil C18 column（ 4. 6 mm × 250 mm,5 μm） ,mobile phase：Acetonitrile-Water （ v/v） =30∶ 70,the detection wavelength at 270 nm,the flow rate at 1. 0 ml.min-1. RESULTS：The standard curve was linearover the range of （ 5. 35 -85. 6） μg.ml -1（ r = 0. 9998） ,and the average recovery was 98. 06 with RSD of 0. 44% （ n = 6）. CONCLU-SION：The method is simple,sensitive and accurate,and available for control of Xianlexiong Capsules.