血小板源性伤口愈合因子促糖尿病大鼠伤口愈合与成纤维细胞前胶原Ⅰ型(α_1)基因表达的关系

ACCELERATION OF WOUND HEALING IN DIABETIC RATS BY PDWHF AND ITS RELATION WITH ITS ACTIVITY TO STIMULATE PROCOLLAGEN Ⅰ (α 1) GENE EXPRESSION

目的研究外源性血小板源性伤口愈合因子（ＰＤＷＨＦ）对糖尿病大鼠伤口愈合的作用及其机理。方法通过糖尿病大鼠背部成对切口伤模型证实ＰＤＷＨＦ应用于伤口局部的情况。结果伤后７、１０及１４天，糖尿病治疗组及糖尿病自身对照组伤口抗张力值分别为１００．９４±１２．７４、２４４．０２±４８．０２、５３５．０８±３６．２６和６５．６６±１１．７６、１１０．７４±２７．４４、２７１．４６±２９．８０Ｎ／ｍ，两组间差异显著（Ｐ＜０．０１）。体外实验发现，生长融合的伤口成纤维细胞与ＰＤＷＨＦ孵育４、８及１２小时后，ＰＤＷＨＧ组前胶原Ⅰ型（α１）ｍＲＮＡ分别较对照组高０．９、３．７及２．２倍。

Objective The effect of platelet derived wound healing factor (PDWHF) on wound healing in diabetic rats was studied. Methods Forty four male SD rats were randomly divided into 2 groups. Thirty two rats of experimental group accepted intraperitoneal injection of alloxan (1.5 mg/10 g body weight). Within one or two days after injection, while the blood sugar of the rats was higher than 180 mg/dl, the animal model of diabetic rat should have been established. Then a dorsal incision was given to every rat. After the addition of PDWHF (the experimental group) or bovine albumin (the control group), the incision was sutured up. Seven, ten and fourteen days after operation, the breaking strength of the wound was measured. On another hand, specimen from the wound was taken for the culture of fibroblasts. When the cultured fibroblasts have been incubated with 10% PDWHF for 4、8 and 12 hours, the procollagen Ⅰ(α 1) mRNA levels were examined respectively, and compared with those of control. Results Significant difference in wound breaking strength had been observed between PDWHF treated incisions and the control on 7、 10 and 14 days after wounding (P<0.01). Experiment in vitro demonstrated that the procollagen Ⅰ (α 1) mRNA levels in wound fibroblasts incubated with 10% PDWHF for 4、 8 and 12 hours were 0.9、 3.7 and 2.2 folds higher than those in fibroblasts in control. Conclusion It was suggested that direct stimulation of procollagen Ⅰ (α 1) gene expression was one of the ways that PDWHF played its role in accelerating wound healing.