益气活血系列方对膜性肾炎模型基底膜影响的实验研究

Experimental Study of Influence of Yiqi Huoxue Serial Recipes on Basement Membrane in Membranous Nephritis in Rabbits

目的：探讨益气活血系列方对膜性肾炎的治疗作用机理。方法：以阳离子牛血清白蛋白（ＣＢＳＡ）造成家兔膜性肾炎模型，分为清热膜肾冲剂组（中药Ⅰ组）、补肾膜肾冲剂组（中药Ⅱ组）、激素组、对照组和正常组。每周测定１次２４ｈ尿蛋白，实验结束时测定血浆白蛋白、血脂、肾功能、前列腺素等，并做光镜、电镜、免疫荧光以及１％聚乙烯亚胺染色进行基底膜电荷检测。结果：中药Ⅰ组、中药Ⅱ组的２４ｈ尿蛋白定量、血浆白蛋白、血脂均较对照组明显改善（Ｐ＜００１或Ｐ＜００５），而激素组与对照组比较无明显差异（Ｐ＞００５），且中药Ⅰ组２４ｈ尿蛋白及血脂与激素组比较有明显降低（Ｐ＜００５）。光镜、电镜、免疫荧光及电荷测定均发现用药组较对照组病理损害减轻。结论：益气活血系列方能降低尿蛋白，提高血浆白蛋白，恢复肾小球基底膜电荷屏障和促进免疫复合物的清除。

Objective: To clucidate the mechanism of the therapeutic effect of Yiqi Huoxue (YQHX) serial recipes on membranous nephritis. Methods: Forty nine New Zealand male rabbits were made to menbranous nephritis model by cation bovine serum albumin and divided into 5 groups, the group A (treated by Qingre Moshen granule), B (treated by Bushen Moshen granule), C (treated by steroid), D (the control group) and E (the normal group). Twenty four hours' urinary protein content of the animals was determined every week, and plasma albumin, blood lipid, renal function and prostaglandins were tested by the end of experiment. And pathological changes of basement membrane were observed by using light, electronic and immunofluorescent microscopy with polyethylene imine stain. Results: The 24 hours urinary protien content, plasma albumin and blood lipid in the group A and B were lower than those in the control group significantly, P<0 01 or 0.05, while those in the group C and the D were similar, P>0 05. In comparing the group A and B with the group C, the difference was also significant, P<0 05. Light, electronic and immunofluorescent microscopic examination all showed that the pathologic changes in the group A, B and C were lesser than that of the control, the effect was in the order A>B>C. Conclusion: YQHX serial recipes can reduce urinary protein content, elevate plasma albumin level, restore the charge barrier effect of and attenuate the immnue complex deposition on the basement membrane of glomeruli.