复方活血汤对再生障碍性贫血小鼠骨髓造血细胞粘附分子及细胞周期蛋白表达作用

Effect of Composite Blood Activating Decoction on Expression of Adherent Molecule and Cyclin in Bone Marrow Hematopoietic Cells in Mice of Immune Induced Aplastic Anemia

目的：探讨复方活血汤（简称中药）对免疫诱导的再生障碍性贫血（再障）小鼠骨髓造血细胞粘附分子及细胞周期蛋白表达的作用。方法：建立免疫诱导的再障小鼠模型，喂饲１００％复方活血汤每次０２ｍｌ，每日２次，第１３日用流式细胞仪检测小鼠骨髓单个核细胞β１整合素家族（ＣＤ４９ｄ／ＣＤ２９，α４β１；又称ＶｅｒｙＬａｔｅＡｎｔｉｇｅｎｓ，ＶＬＡ家族）及细胞周期蛋白Ｄ２的表达水平。结果：中药组ＣＤ４９ｄ表达明显高于再障组（Ｐ＜００５），且与正常组无明显差异；中药组细胞周期蛋白Ｄ２表达明显高于再障组（Ｐ＜００１）；而其Ｇ０＋Ｇ１期细胞数显著低于再障组（Ｐ＜００１）。结论：复方活血汤能增强免疫再障小鼠骨髓造血细胞粘附分子ＣＤ４９ｄ和细胞周期蛋白Ｄ２的表达，促进造血细胞的增生。

Objective: To study the effect of composite blood activating decoction (CBAD) on expression of adherent molecule CD 49 d and cyclin D 2 in bone marrow hematopoietic cells in experimental immune mediated aplastic anemia (AA) mice. Methods: After the model of AA was established, the animals were fed with 0.2ml of 100% CBAD, twice a day for 12 days. Te CD 49 d and cyclin D 2 expression level of bone marrow hematopoietic cells in model mice were measured by flow cytometor analysis system at the 13th day of experiment. Results: CD 49 d expression level in CBAD group was significantly higher than that in the AA group (P<0 05), and was similar to that in the normal group (P>0 05). The expression of cyclin D 2 in CBAD group was significantly higher than that in the AA group (P<0 01), but the cell count of G 0+G 1 phase was significantly lower in the CBAD group, as compared with the AA group, P<0 01. Conclusion: CBAD can increase the expression of adherent molecule CD 49 d and cyclin D 2 in bone marrow hematopoietic cells, and promote the growth of hematopoietic cells.