丹参有效部位(丹参-9403)对骨折愈合影响的生物力学实验研究

Biomechanical Experimental Study on Effective Fraction of Radix Salviae Miltiorrhizae on Healing of Bone Fracture

目的：探讨丹参提取的有效部位（丹参－９４０３）对骨折愈合的影响。方法：用Ｗｉｓｔａｒ大白鼠进行标准骨折（桡骨中１／３）造模后，随机分为４组（即丹参－９４０３、金葡液、生理盐水及丹参注射液组），术后第２天开始分别灌胃各组药物，连续喂养２５、３９、５０天后处死，取完整桡骨，置于岛津Ａｕｔｏｇｒａｐｈ万能材料测试仪上测定３点抗折强度的载荷、应力。结果：丹参－９４０３组在３９天的载荷指标和２５、３９、５０天应力指标最高，分别为（６２０±１３２）牛顿（Ｎ），（５７１±３５８）Ｎ／ｍｍ２、（８２７±２４２）Ｎ／ｍｍ２、（６６２５±２６５１）Ｎ／ｍｍ２，与其他各组比较，有显著性差异（Ｐ＜００５）。结论：丹参－９４０３具有提高骨折断端强度的作用。

Objective: To assess the effect of Danshen 9403(DS 9403, an effective fraction of Radix Salviae Miltiorrhizae) on healing of bone fracture. Methods: Standardized radial fracture was performed in 120 Wistar rats. The model animals were randomized into four groups: group A was fed with DS 9403, group B injected with Staphylococcus Aureus, group C with normal saline, and group D with Salviae injection. The treatment began at the first day of fracture. The rats were sacrificed on the 25th, 39th and 50th day separately in batches and their intact radii were removed by dissection for detecting load and stress of three point bending test with autograph universal material testing machine (Shimazu, Japan). Results: The parameters of load in DS 9403 treated group on the 39th day and that of stress at 25th, 39th and 50th day were (6 20±1 32)N, (5 71±3 58)N/mm 2、(8.27±2.42)N/mm 2 and (66.25±26.21)N/mm 2 respectively, which were significantly higher than those in other groups, P<0 05. Conclusion: DS 9403 has the action of increasig the strength of fracture healing site.