静脉注射内毒素建立麻醉状态下绵羊急性呼吸窘迫综合征模型

Establishment of an acute respiratory distress syndrome model in sheep under anesthesia state by intravenous lipopolysaccharide challenge

目的：探讨建立静注内毒素致麻醉状态下绵羊急性呼吸窘迫综合征（ＡＲＤＳ）模型的方法。方法：绵羊静脉给予内毒素（ＬＰＳ）３μｇ／ｋｇ，３０分钟匀速泵入。观察血流动力学、肺力学参数和肺气体交换的变化。结果：１２只绵羊中有８只在３小时内发生ＡＲＤＳ。第４～８小时间血流动力学（心脏指数、平均血管阻力指数、肺血管阻力指数、氧输送）、肺力学参数（肺动态顺应性、平均气道阻力、气道峰压值、气道阻力）、肺气体交换（改良氧合指数、动脉氧分压）均平稳（Ｐ均＞０．０５）。结论：静注ＬＰＳ能够复制出稳定时间较长的麻醉状态下绵羊ＡＲＤＳ模型。

Objective:To establish an acute respiratory distress syndrome (ARDS)model in sheep under anesthesia state by intravenous lipopolysaccharide(LPS) challenge.Methods:Sheep was infused intravenously with LPS (3 μg/kg) within 30 minutes,and parameters of hemodynamics,lung mechanics and gas exchange were observed during the experiment.Results:8 of 12 sheep developed typical ARDS within 3 hours following LPS challenge.All parameters were kept constantly from the fourth to the eighth hour (all P >0 05), inclu ding hemodynamics (CI,O 2,PVRI,SVRI),lung mechanics (dynamic compliance,resistance,peak inspiratory pressure,mean airway pressure) and gas exchange (PaO 2/FiO 2,PaCO 2).Conclusions:A prolonged ARDS sheep model could be set up by intravenous LPS challenge under anesthesia state.