沙葱种子盐溶蛋白电泳技术体系构建

STUDY ON PAGE OF LSOLATIONG SEEDS SALT-SOLUBLE PROTEINS OF Allium mongolicum RRGEL.

以沙葱种子为材料,利用PAGE的方法,探索了沙葱种子盐溶蛋白分离的适宜实验条件。其结果表明分离沙葱盐溶蛋白的PAGE电泳的适宜条件为:30%H2O2/1mL,胶浓度为13%,胶液为5μL,交联度为4%,加样量为20μL和25μL,电极液pH值是3.5,电泳方式为先低后高再低。

The experiment was conducted to optimize the conditions of PAGE for isolating salt-soluble proteins of Allium mongolicum Rrgel..seeds.The optimal conditions for PAGE w were as follows.The result showed that 30% H2O2 5μl,gel gel concentration 13%,cross-linkages 4%,pH of gel buffer 3.5,and the injecting volumes is 20μl and 25μl.