鲫鱼尾鳍细胞培养及冻存与复苏的初步研究

CARP TALL FIN TISSUE CULTURE AND THE PRELLMINARY STUEY OF CRYOPRESERVATION AND RECOVERY

本文主要从鱼类种质资源保存的目的出发,一方面以鲫鱼尾鳍组织为材料,进行细胞培养,并对所获得的原代和传代细胞进行形态学观察;另一方面以传代细胞为材料,采用逐步降温法和悬于液氮罐口两种方法冻存细胞,每种方法均以甘油和DMSO为冻存剂,通过计算复苏率,找到最适宜的冻存方法与冻存剂。实验结果:成功培养了鲫鱼尾鳍细胞,现已传至第7代;细胞在传代过程中呈成纤维细胞增多的趋势;采用逐步降温法和使用DMSO为冻存剂细胞复苏率较高。

This research aims at fish germ plasm preservation.Firstly,by observing the result from the experiment of culturing the cells of crucian carp fin tissue,morphological features of primary cultured and sub-cultured cells were examined.Secondly,by calculation and assessing the cell recovery results of storing sub cultured cells by either gradually cooling down or hanging in the liquid nitrogen tank,the better cryopreservation method and suitable antifreeze were determined.In the test,glycerin and DMSO were used as antifreeze.The result shows that the crucian carp fin tissue cells were successfully cultured into the 7th generation and that there is a growing tendency of increasing fibroblast when transferring cells.Higher recovery rate were also happened when gradual cooling down and DMSO were employed.The result shows that the crucian carp fin tissue cells were successfully cultured into the 7th generation and that there is a growing tendency of increasing fibroblast when transferring cells.Higher recovery rate were also happened when gradual cooling down and DMSO were employed.