摘要
本文以鄂尔多斯高原碱湖钝顶节旋藻A1、鄂尔多斯节旋藻A2、方胞节旋藻A3、引进的非洲Chad湖钝顶节旋藻A4和墨西哥Texcoco湖极大节旋藻A5为实验材料,采用CTAB-溶菌酶法提取其基因组DNA,紫外分光光度法测定DNA含量。结果表明:5个节旋藻样品的DNA含量在3.1184mg/g DW^19.9076mg/g DW范围内,含量高低排列是A4>A2>A1>A5>A3,不同样品DNA含量存在较大的差异。
The genomic DNA of five species in Arthrospira was extracted by CTAB-lysozyme method and contents of DNA were determined by means of ultraviolet spectrophotometer,which include Arthrospira platensis（A1）,A.erdosensis（A2）,A.jenner（A3） from alkaline lakes in Erdos Plateau,A.platensis（A4） from Chad Lake and A.maxima（A5） from Texcoco Lake in Mexico.The results indicate that the content of DNA in 5 species in Arthrospira are 3.1184～19.9076 mg/g DW.Following tendency is A4A2A1A5A3.There are obvious differences in the content of DNA between 5 species.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2009年第3期241-243,共3页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金(39960062)
内蒙古自治区高等学校科学研究项目(NJzy08025)
关键词
节旋藻
DNA含量
紫外分光光度法
Arthrospira
content of DNA
ultraviolet spectrophotometer
