猪氟烷基因型的PCR检测及杂合子氟烷阳性猪的发现

Detection of Halothane Genotype with PCR RFLP and Discovery of Halothane Positive Pig with Heterozygote

对１４７头猪进行了氟烷测验，测得氟烷阳性猪３头，阳性率为２．０４％。运用ＰＣＲ－ＲＦＬＰ分析法对氟烷阳性猪及其同胞进行了基因型检测，结果４头被测猪中有２头为隐性纯合子，２头为杂合子。结合氟烷测验结果，２头隐性纯合子表现为氟烷阳性，符合一般规律；而２头杂合子中发现１头为氟烷阳性，此前尚未见报道。

Halothane test (HT) was carried out on 147 pigs. Three pigs expressed halothane positive (HP) with the HP rate of 2.04%. The genotypes of HP pigs and their siblings (total 4 pigs) were detected with PCR RFLP. 2 of them were recessive homozygote (nn), and the other 2 were heterozygote (Nn). With the comparision of the HT and PCR RFLP, 1 of 2 Nn pigs was found to be HP, which had not been reported before indicating that there is no complete correspounding relationship between the phenotype and genotype of halothane.