云南龙陵黄山羊线粒体DNA限制性酶切分析

Restriction Cleavage Analysis of mtDNA from Yunnan Red Goat

采用碱性变性法提取来自于龙陵县不同地区的１８只黄山羊个体的线粒体ＤＮＡ（ｍｔＤＮＡ），并用ＡｐａⅠ，ＡｖａⅠ，ＢａｍＨⅠ，ＢｃｌⅠ，ＢｇｌⅠ，ＢｇｌⅡ，ＣｌａⅠ，ＤｒａⅠ，ＥｃｏＲⅠ，ＥｃｏＲⅤ，ＨａｅⅠ，ＨｉｎｄⅢ，ＫｐｎⅠ，ＰｓｔⅠ，ＰｖｕⅡ，ＳａｃⅠ，ＳａｌⅠ，ＳｍａⅠ，ＳｔｕⅠ和ＸｈｏⅠ等２０种限制性内切酶进行酶切分析．结果发现龙陵黄山羊线粒体ＤＮＡ的分子量大小约为１５８Ｋｂ；不同酶的酶切位点分别为：ＤｒａⅠ有７个酶切位点，ＡｖａⅡ有６个酶切位点，ＥｃｏＲⅤ和ＳｔｕⅠ共有５个酶切位点，ＨｉｎｄⅢ和ＨｅａⅡ有４个酶切位点，ＢａｍＨⅠ，ＢｇｌⅡ，ＰｓｔⅠ和ＰｖｕⅡ有３个酶切位点，ＡｐａⅠ，ＣｌａⅠ有２个酶切位点，其余有１个酶切位点．

Mitochondral DNAs of Yunnan red goat distributed in the different regions of Longling county of Yunnan province has been digested with twenty restriction endonucleases,i.e.:Apa ,Ava ,BamH ,Bcl ,Bgl ,Bgl ,Cla ,Dra ,EcoR ,EcoR ,Hae ,Hind ,Kpn ,Pst ,Pvu ,Sac ,Sal ,Sma ,Stu and Xho .The molecular weight of the fragements is about 158kb.mtDNAs were broken into different sizes by the different restriction endonucleases .There are 7 fragments for Dra ,6 for Ava ,5 for Ecor and Stu ,4 for Hind and Hae ,3 for BamH ,Bgl ,Pst and Pvu ,2 for Apa and Cla ,and 1 for the otheres.