摘要
以蚜虫传染发生云南烟草丛枝症病害的烟株为材料进行组织培养,连续培育3代,以第3代组培烟苗为毒源经蚜虫传染健康烟株,提取毒源和各实验烟株的总核酸进行琼脂糖凝胶电泳.结果表明:第3代组培烟苗为毒源经蚜虫传染的烟株发病表现丛枝症,并检测到与云南烟草丛枝症病害相关的低分子量RNA.说明与云南烟草丛枝症病害相关的病原能在寄主离体培养组织中长期保存,成功建立云南烟草丛枝症病害的标准毒株.
The tissue culture of the infected tobaccos by aphids continued three
generations.The total nucleic acid from the third generation and the infected tobacco inoculated
with the pathogen of the third generation by aphids was electrophoresis on agarose gel.The
showed:the infected tobacco inoculated with the pathogen of the third generation by aphids
appeared withches'broom symptom in which the Tobacco witches'broom symptom diseases
associated-RNA could be detected.So we succeed to preserve the pathogen in long-term in the
tissue culture of host and found the standard stain of tobacco witches'broom symptom disease
in Yunnan.
出处
《云南农业大学学报》
CAS
CSCD
1999年第1期96-98,共3页
Journal of Yunnan Agricultural University
基金
云南省应用基础研究基金
云南省烟草科研基金
关键词
云南
烟草丛枝症病害
组织培养
标准毒株
Tobacco witches'broom symptom diseases
Tissue culture
Standard
strain