云南烟草丛枝症病害研究 IV建立标准毒株

Studies on Tobacco Witches' Broom SymptomDiseases in YunnanIVFound the Standard Strain

以蚜虫传染发生云南烟草丛枝症病害的烟株为材料进行组织培养，连续培育３代，以第３代组培烟苗为毒源经蚜虫传染健康烟株，提取毒源和各实验烟株的总核酸进行琼脂糖凝胶电泳．结果表明：第３代组培烟苗为毒源经蚜虫传染的烟株发病表现丛枝症，并检测到与云南烟草丛枝症病害相关的低分子量ＲＮＡ．说明与云南烟草丛枝症病害相关的病原能在寄主离体培养组织中长期保存，成功建立云南烟草丛枝症病害的标准毒株．

The tissue culture of the infected tobaccos by aphids continued three generations.The total nucleic acid from the third generation and the infected tobacco inoculated with the pathogen of the third generation by aphids was electrophoresis on agarose gel.The showed:the infected tobacco inoculated with the pathogen of the third generation by aphids appeared withches'broom symptom in which the Tobacco witches'broom symptom diseases associated-RNA could be detected.So we succeed to preserve the pathogen in long-term in the tissue culture of host and found the standard stain of tobacco witches'broom symptom disease in Yunnan.