免疫介导型再生障碍性贫血小鼠间充质干细胞成骨及成脂潜能改变

The Potential Alteration of Their Osteogenesis and Adipocyte Differentiation in Bone Marrow Mesenchymal Stem Cells from Mice with Immune-mediated Aplastic Anemia

[目的]探讨免疫介导型再生障碍性贫血小鼠间充质干细胞(msenchymal stem cell,MSC)向成骨及成脂肪细胞分化潜能的改变。[方法]采用免疫介导法进行再障造模,Babl/c小鼠经60Co射线亚致死剂量照射后,自尾静脉输入DBA/2小鼠的淋巴细胞建立免疫介导型再生障碍性贫血小鼠模型;造模15天后进行再生障碍性贫血小鼠骨髓间充质干细胞培养,并检测骨髓成纤维细胞集落形成单位(CFU-F)数量变化,观察模型小鼠骨髓病理改变;茜素红和油红O分别检测模型小鼠MSC钙结节数和脂肪细胞形成率。[结果]再障模型小鼠CFU-F数明显减少;骨髓病理切片显示,造血组织减少,并脂肪化;再障小鼠MSC钙结节数量减少,脂肪细胞分化率显著增加。[结论]免疫介导再生障碍性贫血小鼠MSC成骨潜能减弱,成脂潜能明显增强。

[Objective]To explore the potential alteration of their osteogenesis and adipocyte differentiation in bone marrow mesenchymal stems cells from mice with immune-mediated aplastic anemia.[Methods]Balb/c mice model of immune-mediated aplastic anemia was established by radiation with sublethal dose of 60Co following the intravenously infusion lymphocytes of DAB/2 mice.The culture of the MSC and CFU-F and pathological examination of bone marrow were carried out 15 days later.The amount of calcium node and the frequency of adipocyte differentiation were evaluated respectively by alizarin red and oil red O.[Result]The number of CFU-F and the number of calcium node in model mice decreased more greatly than normal mice,but the ferenqucy of adipocyte differentiation was more increased greatly in model mice than normal mice;the pathological examination showed in the model mice,the hematopoietic structure was destroyed and filled with abundant adipocyte.[Conclusion]The potention of osteogenesis and adipocyte differentiation was altered in the mice with immune-mediated aplastic anemia.