暗纹东方鲀生长激素基因克隆与同源性分析

Molecular Cloning and Sequence Analysis of Takifugu fasciatus GH gene

克隆获得暗纹东方鲀GH基因,该基因包含6个外显子和5个内含子,在此基础上获得其cDNA序列,共有591bp,编码196个氨基酸。将该cDNA序列转换成蛋白质序列后,结合NCBI数据库中得到的其它24种脊椎动物生长激素基因的蛋白质序列,运用MEGA3.1,以UPGMA法构建东方鲀等25种脊椎动物的生长激素基因进化树,14种鱼类中除了鳗鲡和鳝鱼外聚为一大类,其它动物中哺乳动物、反刍动物、家禽、爬行动物、人以及鳗鲡等聚为另一大类,暗纹东方鲀与红鳍东方鲀生长激素基因的氨基酸同源性达到100%,与斗鱼、杜父鱼、金鲈生长激素基因的氨基酸同源性达到70%以上,与人的生长激素基因的氨基酸同源性也达到40%;斑马鱼、草鱼、鲋鱼以及鲤鱼之间生长激素基因的氨基酸同源性在85%以上;哺乳动物、反刍动物、家禽的生长激素基因的氨基酸同源性在90%左右。从进化树的结果来看,与传统分类学研究结果基本一致,为进一步研究GH基因在胚胎发育及个体生长中的功能奠定基础。

GH gene in Takifugu fasciatus was cloned,and there were 6 exons and 5 introns in the GH gene,196 amino acids were coded.The sequences of cDNA were brought out based on the structure of GH gene.The acid sequences of GH gene in Takifugu,combined with other 24 vertebrate species were obtained from the Gene Bank,and the phylogenetic tree of GH gene was constructed with MEGA 3.1 using UPGMA method.In the UPGMA consensus tree,25 species were divided into two clusters.The results were found agreeing with those by traditional classification,Which establishes a good foundation for further studies on GH gene functions in the embryonic development and the body growth.